GlycoPlants

The N-glycan database to early detect Crop’s diseases and nutrient deficiencies

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Research Group of Applied Plant Glycobiology, Dama Research Center limited

GlycoPlants is a project with the purpose of building up an Open Access database to store and share glycan retention properties of structures that are conditionally associated with plant stress caused by nutrient deficiency or crop’s diseases

 

Problem we focus on

Currently ther is a negligible amount of open access Glycan attributes such as profiles available in the literature, (e.g only 4 glycan profiles detected in plant samples are published in the GlycoStore database, which is widely accepted in the field). That’s unfortunate, since glycosylation is very important in plant science  considering that it can modify various biological functions of proteins, and affect growth, physiology, and development of crops. It seemed to be confirmed by our basic research in which we found statistical correlations between nutrient deficiency symptoms of crops  and changes in suspected PCT-FNGs profiles based only on GU values, observed by CE-LIF.

 

 

Beta

◀️Responsive Database I Slide screen for more records ▶️

As of current GlycoPlants consists: Suspected structures being linked to reactions to stress caused by nutrient (N) deficiency in plants based on our separation in tomato xylem sap; and exact GU matches consulted with GlycoStore.org; and Complex type free N-glycan detected mostly by HPLC (extracted from literature, please find DOI(s) in Reference column; and we periodically extract plant data (if any) from Glycostore. Last Update: 18 May, 2020

IDStructureGUGlycanRT TypeAssociated diseases/nutrient deficiencysampleSample ageVariotypeOriginReferencesEquipmentDetector typeBuffer Separation temperature (℃)Lead researcher InstitutionContact
1A3G(4)3S(3,6,3,6)44.19CE N deficiencyTomato 55 days old seedlingsSolanum lycopersicumEcuadorDRC preliminaryProteomelab CE, with Laser induced fluorescence detector module405 nm diode laser. Cartridge with 50 cm effective length (60 cm total length) bare fused silica (BFS) capillary with 50 µm inner (ID) diameter. Filtration: 520 nm bandpass (Beckman-Coulter, Fullerton, CA, USA)HR-NCHO separation gel30Mátyás,BResearch Group of Applied Plant Glycobiology, Dama Research Center limited bmatyas@edu.damaresearch.com
2A3G(4)3S(6,6,3)34.92n.a.CEN deficiencyTomato 55 days old seedlingsSolanum lycopersicumEcuadorDRC preliminaryProteomelab CE, with Laser induced fluorescence detector module405 nm diode laser. Cartridge with 50 cm effective length (60 cm total length) bare fused silica (BFS) capillary with 50 µm inner (ID) diameter. Filtration: 520 nm bandpass (Beckman-Coulter, Fullerton, CA, USA)HR-NCHO separation gel30Mátyás,BResearch Group of Applied Plant Glycobiology, Dama Research Center limited bmatyas@edu.damaresearch.com
3A2BG29.84CEN deficiencyTomato 55 days old seedlingsSolanum lycopersicumEcuadorDRC preliminaryProteomelab CE, with Laser induced fluorescence detector module405 nm diode laser. Cartridge with 50 cm effective length (60 cm total length) bare fused silica (BFS) capillary with 50 µm inner (ID) diameter. Filtration: 520 nm bandpass (Beckman-Coulter, Fullerton, CA, USA)HR-NCHO separation gel30Mátyás,BResearch Group of Applied Plant Glycobiology, Dama Research Center limited bmatyas@edu.damaresearch.com
4G1F1GN2M3FXn.a.HPLCn.a.Tomato 55 days old seedlingsKGM 172, Kagome Co. Tochigi, JapanJapan10.1080/09168451.2019.1608803RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)Filtration: Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,n.a.n.a.Yoshinobu KimuraGraduate School of Environmental and Life Science, Okayama Universityjosh8mar@okayama-u.ac.jp
5G1GN2M3FXn.a.HPLCn.a.Tomato 55 days old seedlingsKGM 172, Kagome Co. Tochigi, JapanJapan10.1080/09168451.2019.1608803RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)Filtration: Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,n.a.n.a.Yoshinobu KimuraGraduate School of Environmental and Life Science, Okayama Universityjosh8mar@okayama-u.ac.jp
6GN2M3FXn.a.HPLCn.a.Tomato 55 days old seedlingsKGM 172, Kagome Co. Tochigi, JapanJapan10.1080/09168451.2019.1608803RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,n.a.n.a.Yoshinobu KimuraGraduate School of Environmental and Life Science, Okayama Universityjosh8mar@okayama-u.ac.jp
7M3FXn.a.HPLCn.a.Tomato 55 days old seedlingsKGM 172, Kagome Co. Tochigi, JapanJapan10.1080/09168451.2019.1608803RP-HPLC; usingCosmosil 5C18 AR column (6.0 x 250 mm, Nacalai Tesque, Kyoto)Gel with Sephadex G-25 superfine column (1.5 x 16 cm) in 0.1 N NH4OH,n.a.n.a.Yoshinobu KimuraGraduate School of Environmental and Life Science, Okayama Universityjosh8mar@okayama-u.ac.jp

 

 

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